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Entry information : RopHCMDn (pcaL)
Entry ID 4562
Creation 2007-02-15 (Filippo Passardi)
Last sequence changes 2007-02-15 (Filippo Passardi)
Sequence status complete
Reviewer Christophe Dunand
Last annotation changes 2010-05-06 (Christophe Dunand)
Peroxidase information: RopHCMDn (pcaL)
Name (synonym) RopHCMDn (pcaL)
Class Hydrolase-CMD fusion (no peroxidase activity)    [Orthogroup: HCMDn001]
Taxonomy Bacteria Actinobacteria Actinobacteria (class) Nocardiaceae Rhodococcus
Organism Rhodococcus opacus (Nocardia opaca)    [TaxId: 37919 ]
Cellular localisation N/D
Tissue type N/D
Inducer N/D
Repressor N/D
Best BLASTp hits
Perox score E-value RopHCMDn
start..stop		 S start..stop
RHsHCMDn_DK17 786 0 1..400 1..400
RHsHCMDn_RHA1 782 0 1..400 1..400
ReHCMDn_H16 306 5.24e-102 5..386 11..387
ScoHCMDn01 291 2.07e-96 1..385 1..368
Literature and cross-references RopHCMDn (pcaL)
Literature Eulberg,D., Lakner,S., Golovleva,L.A. and Schlomann,M. Characterization of a protocatechuate catabolic gene cluster from Rhodococcus opacus 1CP: evidence for a merged enzyme with 4-carboxymuconolactone-decarboxylating and 3-oxoadipate enol-lactone-hydrolyzing activity. J. Bacteriol. 180 (5), 1072-1081 (1998)
Protein ref. GenPept:   AAC38246 UniProtKB:   O67982
DNA ref. GenBank:   AF003947
Protein sequence: RopHCMDn (pcaL)
Sequence Properties
first value : protein
second value (mature protein)
Length (aa):   %s   400
PWM (Da):   %s   42103.12 Transmb domain:   %s   o303-321i442-464o484-506i676-693o
PI (pH):   %s   4.78
Sequence
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*.........1 .........2 .........3 .........4 .........5 .........6 .........7 .........8 .........9 .........0 .........1 .........2
MTVALAHEIS GPRNGAADAP VVVLLGSLGS NRSMWDPQIA ALSDECRVVA VDQRGHGESP APDGPYSVRD LSEDVLALLD SLGVDAAHFV GLSMGGAIAQ WLGAHTPRRV LSLSLLCTAA  KFGEPQAWTE RAAASRTDGP ESLADAVVAR WFSEGFAKRD PEFVRHYREM IASTSPEGYA ACCDALADWD FTADLSRISA PTLVIAGEED PSTPPSVMQI LADGITGARF EVLSPAAHVA  NLEQAGAVTA LLREHIAGGG YARGRRAAHA QGMTVRRSVL GDAHVDRSVA GTTDFTAPFQ DFITRTAWGD IWSRPGLDHE LRRLLTIAVL TAVGNEHELD MHIRAALRAG VDADTIGEVL 
LHTAVYAGVP NSNLGFALGK QALADLSSTE TGATEENSQT 

Retrieve as FASTA  
Remarks Complete sequence from genomic. Strain="1CP". The full fusion protein has been isolated (see Literature). Member of protocatechuate-degrading gene clusters (pcaI', pcaH, pcaG, pcaB, pcaL, pcaR, and pcaF').