Entry information : SceKat01 (SceKatA / CTA1 / catalase A)
Entry ID 5175
Creation 2007-04-12 (Marcel Zamocky)
Last sequence changes 2007-04-12 (Marcel Zamocky)
Sequence status complete
Reviewer Christophe Dunand
Last annotation changes 2012-04-27 (Catherine Mathe (Scipio))
Peroxidase information: SceKat01 (SceKatA / CTA1 / catalase A)
Name (synonym) SceKat01 (SceKatA / CTA1 / catalase A)
Class Catalase    [Orthogroup: Kat001]
Taxonomy Eukaryota Fungi Ascomycota Saccharomycetes Saccharomycetaceae Saccharomyces
Organism Saccharomyces cerevisiae    [TaxId: 4932 ]
Cellular localisation Peroxisomal
Tissue type N/D
Inducers Carbon starvation
Oleate
Repressor glucose-rich medium
Best BLASTp hits
Perox score E-value SceKat01
start..stop
S start..stop
KlKat02 869 0 11..501 5..496
CglKat01 867 0 9..507 4..501
AgoKat01 853 0 4..503 1..499
DhKat02 696 0 21..501 4..478
Gene structure Fichierperl './assets/cgi-bin/draw_exon.pl' '5175' 'complement(join(968130..969677))' Exons
ExonStart..EndSize ExonStart..EndSize ExonStart..EndSize ExonStart..EndSize
N° 1 968130..969677 1546  
complement(join(968130..969677))


exon

Literature and cross-references SceKat01 (SceKatA / CTA1 / catalase A)
Literature Mate MJ, Zamocky M, Nykyri LM, Herzog C, Alzari PM, Betzel C, Koller F, Fita I "Structure of catalase-A from Saccharomyces cerevisiae." J. Mol. Biol. 286: 135-149 (1999); Cohen G, Rapatz W, Ruis H "Sequence of the Saccharomyces cerevisiae CTA1 gene and amino acid sequence of catalase A derived from it." Eur. J. Biochem. 176:159-163 (1988)
Protein ref. UniProtKB:   P15202
DNA ref. JGI genome:   chrIV (969677..968130)
Protein sequence: SceKat01 (SceKatA / CTA1 / catalase A)
Sequence Properties
first value : protein
second value (mature protein)
Length (aa):   %s   515
PWM (Da):   %s   58371.85  
PI (pH):   %s   7.29
Sequence
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Send to Peroxiscan
*.........1 .........2 .........3 .........4 .........5 .........6 .........7 .........8 .........9 .........0 .........1 .........2
MSKLGQEKNE VNYSDVREDR VVTNSTGNPI NEPFVTQRIG EHGPLLLQDY NLIDSLAHFN RENIPQRNPH AHGSGAFGYF EVTDDITDIC GSAMFSKIGK RTKCLTRFST VGGDKGSADT  VRDPRGFATK FYTEEGNLDW VYNNTPVFFI RDPSKFPHFI HTQKRNPQTN LRDADMFWDF LTTPENQVAI HQVMILFSDR GTPANYRSMH GYSGHTYKWS NKNGDWHYVQ VHIKTDQGIK  NLTIEEATKI AGSNPDYCQQ DLFEAIQNGN YPSWTVYIQT MTERDAKKLP FSVFDLTKVW PQGQFPLRRV GKIVLNENPL NFFAQVEQAA FAPSTTVPYQ EASADPVLQA RLFSYADAHR  YRLGPNFHQI PVNCPYASKF FNPAIRDGPM NVNGNFGSEP TYLANDKSYT YIQQDRPIQQ HQEVWNGPAI PYHWATSPGD VDFVQARNLY RVLGKQPGQQ KNLAYNIGIH VEGACPQIQQ 
RVYDMFARVD KGLSEAIKKV AEAKHASELS SNSKF 

Retrieve as FASTA  
Remarks Complete sequence from genomic (Chromo 4, no intron). Strain="ATCC 204511 / S288c / AB972".
Structure known - PDB file 1A4E.
Overexpression in yeast Saccharomyces cerevisiae available. Several mutants of this gene with increased peroxidase activity available.
DNA
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.........1 .........2 .........3 .........4 .........5 .........6 .........7 .........8 .........9 .........0 .........1 .........2
ATGTCGAAAT TGGGACAAGA AAAAAATGAA GTAAATTACT CTGATGTAAG AGAGGATAGA GTTGTGACAA ACTCCACTGG TAATCCAATC AATGAACCAT TTGTCACCCA ACGTATTGGG  GAACATGGCC CTTTGCTTTT GCAAGATTAT AACTTAATTG ATTCTTTGGC TCATTTCAAC AGGGAAAATA TTCCTCAAAG GAATCCACAT GCTCATGGTT CTGGTGCCTT CGGCTATTTT  GAAGTAACCG ATGACATTAC TGATATCTGC GGGTCTGCTA TGTTTAGTAA AATTGGGAAA AGAACGAAAT GTCTAACAAG ATTTTCGACT GTGGGTGGTG ATAAAGGTAG TGCCGACACG  GTTCGTGATC CAAGGGGGTT TGCCACCAAA TTCTACACTG AAGAAGGTAA TTTAGATTGG GTCTACAATA ATACACCGGT ATTCTTTATC AGAGACCCTT CCAAGTTCCC TCACTTTATC  CACACACAGA AGAGAAACCC ACAAACCAAC CTAAGGGATG CTGACATGTT TTGGGATTTC CTCACCACTC CTGAAAATCA GGTGGCCATT CATCAAGTAA TGATCCTTTT TTCAGACCGT  GGTACCCCTG CCAACTACCG TAGTATGCAT GGTTATTCTG GTCATACCTA TAAATGGTCC AATAAAAACG GAGATTGGCA TTATGTGCAA GTTCATATCA AAACCGATCA AGGAATAAAG  AATTTGACCA TAGAAGAGGC TACCAAAATT GCGGGATCCA ATCCAGATTA CTGCCAGCAG GATTTATTTG AGGCTATTCA GAATGGAAAC TATCCTTCCT GGACAGTTTA TATTCAAACA  ATGACCGAAC GCGATGCCAA AAAATTACCA TTTTCAGTCT TTGATTTGAC TAAAGTATGG CCTCAGGGGC AATTCCCTTT ACGGCGTGTG GGTAAGATTG TTTTGAACGA GAATCCACTG  AACTTCTTCG CACAGGTGGA ACAAGCTGCC TTCGCCCCCA GTACCACGGT TCCTTACCAA GAAGCAAGCG CTGATCCAGT ATTACAGGCC CGTTTGTTTT CATATGCGGA TGCTCATAGA  TACAGGCTAG GTCCTAACTT CCATCAAATA CCCGTAAACT GTCCATATGC ATCTAAATTT TTCAATCCCG CTATCAGAGA TGGACCGATG AATGTTAACG GCAACTTCGG CTCAGAACCT  ACATATTTGG CCAACGATAA ATCGTACACG TATATCCAAC AGGACAGACC CATTCAACAA CACCAAGAGG TATGGAATGG GCCAGCTATC CCTTATCATT GGGCAACATC CCCAGGTGAT  GTAGATTTCG TGCAAGCAAG AAATCTCTAC CGCGTTTTGG GTAAACAACC TGGACAGCAA AAGAACTTGG CATATAACAT CGGCATTCAT GTAGAAGGCG CCTGTCCTCA AATACAGCAG 
CGCGTTTATG ATATGTTTGC TCGTGTTGAT AAGGGACTAT CTGAGGCAAT TAAAAAAGTA GCTGAGGCAA AACATGCTTC TGAGCTTTCG AGTAACTCCA AATTTTGA 

Retrieve as FASTA  
CDS
Send to BLAST
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ATGTCGAAAT TGGGACAAGA AAAAAATGAA GTAAATTACT CTGATGTAAG AGAGGATAGA GTTGTGACAA ACTCCACTGG TAATCCAATC AATGAACCAT TTGTCACCCA ACGTATTGGG  GAACATGGCC CTTTGCTTTT GCAAGATTAT AACTTAATTG ATTCTTTGGC TCATTTCAAC AGGGAAAATA TTCCTCAAAG GAATCCACAT GCTCATGGTT CTGGTGCCTT CGGCTATTTT  GAAGTAACCG ATGACATTAC TGATATCTGC GGGTCTGCTA TGTTTAGTAA AATTGGGAAA AGAACGAAAT GTCTAACAAG ATTTTCGACT GTGGGTGGTG ATAAAGGTAG TGCCGACACG  GTTCGTGATC CAAGGGGGTT TGCCACCAAA TTCTACACTG AAGAAGGTAA TTTAGATTGG GTCTACAATA ATACACCGGT ATTCTTTATC AGAGACCCTT CCAAGTTCCC TCACTTTATC  CACACACAGA AGAGAAACCC ACAAACCAAC CTAAGGGATG CTGACATGTT TTGGGATTTC CTCACCACTC CTGAAAATCA GGTGGCCATT CATCAAGTAA TGATCCTTTT TTCAGACCGT  GGTACCCCTG CCAACTACCG TAGTATGCAT GGTTATTCTG GTCATACCTA TAAATGGTCC AATAAAAACG GAGATTGGCA TTATGTGCAA GTTCATATCA AAACCGATCA AGGAATAAAG  AATTTGACCA TAGAAGAGGC TACCAAAATT GCGGGATCCA ATCCAGATTA CTGCCAGCAG GATTTATTTG AGGCTATTCA GAATGGAAAC TATCCTTCCT GGACAGTTTA TATTCAAACA  ATGACCGAAC GCGATGCCAA AAAATTACCA TTTTCAGTCT TTGATTTGAC TAAAGTATGG CCTCAGGGGC AATTCCCTTT ACGGCGTGTG GGTAAGATTG TTTTGAACGA GAATCCACTG  AACTTCTTCG CACAGGTGGA ACAAGCTGCC TTCGCCCCCA GTACCACGGT TCCTTACCAA GAAGCAAGCG CTGATCCAGT ATTACAGGCC CGTTTGTTTT CATATGCGGA TGCTCATAGA  TACAGGCTAG GTCCTAACTT CCATCAAATA CCCGTAAACT GTCCATATGC ATCTAAATTT TTCAATCCCG CTATCAGAGA TGGACCGATG AATGTTAACG GCAACTTCGG CTCAGAACCT  ACATATTTGG CCAACGATAA ATCGTACACG TATATCCAAC AGGACAGACC CATTCAACAA CACCAAGAGG TATGGAATGG GCCAGCTATC CCTTATCATT GGGCAACATC CCCAGGTGAT  GTAGATTTCG TGCAAGCAAG AAATCTCTAC CGCGTTTTGG GTAAACAACC TGGACAGCAA AAGAACTTGG CATATAACAT CGGCATTCAT GTAGAAGGCG CCTGTCCTCA AATACAGCAG 
CGCGTTTATG ATATGTTTGC TCGTGTTGAT AAGGGACTAT CTGAGGCAAT TAAAAAAGTA GCTGAGGCAA AACATGCTTC TGAGCTTTCG AGTAACTCCA AATTTTGA 

Retrieve as FASTA