NADH peroxidase/oxidase family

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Last update: 2019-01-31 (Christophe Dunand)

NADH peroxidase (NadPrx)
NADH oxidase (NadOxd)
NADH dehydrogenase (NadDH)


Strong sequence homology exists between NADH peroxidase (NadPrx) and NADH oxidase (NadOxd) which can suggest that both genes originally diverged from a common ancestral sequence. NadPrx adn NadOxd share low level of identity with other members of the flavoprotein disulfide reductase classes. NadPrx as well as other pyridine nucleotide-disulphide reductases (PNDR) use the isoalloxazine ring of FAD to shuttle reducing equivalents from NAD(P)H to a Cys residue that is usually a part of a redox-active disulphide bridge. In a second step, the reduced disulphide reduces the substrate. Particular residues have been found to be highly conserved in within the FAD/NAD binding domain.
NADH peroxidase NADH + H+ +H2O2 2 H2O + NAD+
NADH oxidase
1) 2 NADH + 2 H+ +O2 2 H2O + 2 NAD+
2) NADH + H+ +H+ + O2 H2O2 + NAD+
3) NADH + 2O2 H+ + NAD+ + 2O2.
NADH dehydrogenase


Pfam: PF00070
Pyr_redox; PF07992
Pyr_redox_2; PF02852
Pyr_redox_2 dimerisation domain.
Interpro: IPR001100
Pyr_redox; IPR013027
FAD_pyr_redox; IPR001327
Pyr_redox_NAD_bd; IPR004099

The profiles do not make a distinction between class I, class II oxidoreductases, NADH peroxidase and NADH oxidase


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